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Elevated TNF- levels might impede steroidogenesis under inflammatory conditions and induce germ-cell apoptosis in experimental autoimmune orchitis (Theas et al., 2008)

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NMR chemical shift perturbation analysis The CSPs were calculated according to the following equation: where H and N indicate the chemical shift changes of the amide proton and nitrogen, respectively, using CcpNMR 2.5 49

To quantitatively measure the expression levels of the 60 genes extracted through microarray analysis, RT-qPCR was used